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Lubyani ta tekhnichni kultury

 Vol.6 (11), 2018

ISSN 2307–6429

DOI: 10.48096/btc.2018.6(11).21-28

INDUCTION OF CALUSOGENESIS IN A TECHNICAL (INDUSTRIAL) HEMP IN IN VITRO

 Mishchenko Serhiy
Institute of Bast Crops NAAS

Summary

Separate elements of a technique for introducing hemp into an in vitro culture have been developed. The best option for inducing calusogenesis in technical (industrial) hemp among the studied genotypes in vitro is Murashige and Skoog medium with the addition of 0,5 or 0,3 mg/l 2,4-D, 0,3 mg/l KIN, 0,5 mg/l GA3, vitamins B1, B6, C and 30 g/l sucrose. In this embodiment, the frequency of calusogenesis was 88,5–100%, the formation of green callus with meristematic zones was observed in 73,1–76,5% of the hypocotyl segments, and in some cases organogenesis (shoot formation) also occurred.

Keywords:

industrial hemp, in vitro, variety, phytohormones

Language of the article

Ukrainian

Full Text:

PDF  21-28 (Ukrainian)

References:

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Published:

November 2018

Online:

02.03.2020